Active Recombinant Vaccinia virus B19R Protein, Fc-tagged, Biotinylated

• Cat.No.: B19R-174
• Product Overview: Recombinant Vaccinia virus B19R(His20-Glu351) fused with Fc of Human IgG1 at N-terminal was expressed in HEK293, Biotinylated.

Specification

• Description: B19R is a 60-65 kDa protein encoded by the Vaccinia virus genome and by the genomes of other orthopoxviruses. Its function represents one of several mechanisms used by these viruses to evade the host immune response. It is known as B19R in the Copenhagen strain of Vaccinia and as B18R in the Western Reserve (WR) strain. There is a structurally-unrelated, larger Vaccinia protein that is also known as B18R that contains multiple ankyrin-like repeats. B19R, however, contains three immunoglobulin-like domains and shows homology to human, mouse, and bovine type I interferon receptors. The Wyeth strain of Vaccinia virus encodes a truncated protein that lacks the C-terminal Ig-like domain, and B19R is functionally absent in the Lister strain. B19R functions as a decoy receptor for type I interferons (IFN alpha, beta, omega). It binds to type I interferons from multiple species and prevents IFN signaling through its receptors. B19R binds to the surface of virus infected and uninfected cells where it retains its capacity to bind and neutralize IFN. It shields those cells from the antiviral effects of type I interferons, thereby enabling virus replication and pathogenicity. B19R also limits the effectiveness of IFN alpha produced following TLR activation, and it limits adaptive T cell responses.
• Source: HEK293
• Species: Vaccinia virus
• Tag: Fc
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit Type-I IFN-mediated anti-viral activity. Symons, J.A. et al. (1995) Cell 81:551. The ED50 for this effect, as measured by inhibition of Recombinant Human IFN‑ alpha 2, is 0.3-1.8 ng/mL.
• Molecular Mass: 65 kDa (unlabeled)
• Protein length: His20-Glu351
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Notes: Structure / Form: Disulfide-linked homodimer, biotinylated via sugars
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile
• Reconstitution: Reconstitute at 250 μg/mL in PBS.

Gene Information

• Gene Name: B19R temporal expression: early [ Vaccinia virus ]
• Official Symbol: B19R
• Synonyms: B19R; temporal expression: early
• Gene ID: 3707577
• UniProt ID: P25213