Recombinant Vibrio cholerae serotype O1 CTXB protein, His-tagged

• Cat.No.: CTXB-388V
• Product Overview: Recombinant Vibrio cholerae serotype O1 CTXB protein (Thr22-Asn124), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Cholera enterotoxin subunit B (CTXB) is also known as cholera enterotoxin B chain, cholera enterotoxin gamma chain and choleragenoid. Vibrio cholerae O1 exists as two major serotypes, Inaba and Ogawa, which are associated with the O antigen of the lipopolysaccharide and are capable of unequal reciprocal interconversion. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. CTXB can bind five GM1 gangliosides. Also, it has no toxic activity by itself.
• Source: HEK293
• Species: Vibrio cholerae serotype O1
• Tag: His
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 13.5 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: 103
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: VC1456
• Official Symbol: VC1456
• Synonyms: CTXB, TOXB
• Gene ID: 2613962
• Protein Refseq: NP_231099.1
• UniProt ID: P01556