Recombinant Zebrafish INSB

• Cat.No.: INSB-3604Z
• Product Overview: Recombinant Zebrafish INSB full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Zebrafish
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 oC for short term. For long term storage, store it at -20 oC～-80 oC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: insb preproinsulin b [ Danio rerio (zebrafish) ]
• Official Symbol: INSB
• Gene ID: 566735
• mRNA Refseq: NM_001039064
• Protein Refseq: NP_001034153
• UniProt ID: Q2QAJ9

Reviews

10/04/2022

Replicating outcomes across diverse cell lines underscored the product's consistent behavior.

08/16/2018

The product's adaptability to various buffers contributed to its stable and reliable performance.

07/14/2016

The product's unique attributes propelled our research to innovative heights.

Q&As

What specific DNA region does InsA* and collagenolyzed InsA* bind to?

InsA* and collagenolyzed InsA* bind specifically to a 24-bp region within each of the inverted repeats (IRs) at the ends of IS1.

What is the proposed structure of the transposase of IS1 based on the observations?

The transposase of IS1 is speculated to consist of at least two domains: the N-terminal half, overlapping with InsA, and the C-terminal half, overlapping with B'-InsB. The frameshifting event adds the latter domain to the former, giving transposase activity to recognize IRs and initiate the transposition reaction.

How is the InsA-B'-InsB transframe protein produced from INSB?

The InsA-B'-InsB transframe protein is produced through translational frameshifting at a run of adenines, involving insA and B'-insB reading frames.

What occurs if the frameshifting event does not happen during translation?

If frameshifting does not occur, the InsA protein is produced from INSB.

How does transposase Tnp* bind to DNA sequences with or without IR?

Tnp* binds to DNA sequences with or without IR, but it preferentially binds to sequences with IR.

What was observed in cells with a single adenine insertion in the run of adenines in IS1?

Cells with an IS1 mutant containing a single adenine insertion generated miniplasmids due to efficient production of the InsA-B'-InsB transframe protein, resulting in IS1-mediated deletion.

What is the full name of the protein encoded by INSB?

The protein encoded by INSB is a transframe protein, InsA-B'-InsB.