"VEGF165" Related Products

Recombinant Zebrafish VEGF165 Protein

Cat.No.: VEGF165-1026Z
Product Overview: Recombinant zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified.
Source: Baculovirus-Insect Cells
Species: Zebrafish
Predicted N Terminal: Ala 24
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.01, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-30 ng/mL.
Molecular Mass: The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 KDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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