Active Recombinant Zebrafish VEGF165 Protein

• Cat.No.: VEGF165-1026Z
• Product Overview: Recombinant zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified.

Specification

• Source: Baculovirus-Insect Cells
• Species: Zebrafish
• Predicted N Terminal: Ala 24
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.01, 5%~8% trehalose and mannitol.
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-30 ng/mL.
• Molecular Mass: The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.