SIRT1 Inhibitor/Activator Screening Kit

• Cat.No.: Kit-2156
• Product Overview: In the SIRT1 Inhibitor/Activator screening Kit, Sirtuin 1 deacetylates the substrate, followed by cleavage of the deacetylated substrate to release the fluorescent group, which is detected fluorometrically at Ex/Em = 400/505 nm. In the presence of SIRT1 inhibitor, deacetylation is impeded, preventing cleavage of the substrate and release of the fluorescent group. The SIRT1 activator enhances SIRT1 activity resulting in a higher fluorescent signal in comparison to the control. This kit provides a rapid, simple, sensitive, and reliable test, which is suitable for high-throughput screening of SIRT1 inhibitors/activators. Inhibitor control (Nicotinamide) is included to compare the efficacy of the test inhibitors.

Specification

• Size: 100 assays
• Applications: Screening/characterizing/studying SIRT1 inhibitors and activators
• Storage: Store kit at -20°C, protected from light. Avoid repeated freeze/thaw for all non-buffer components. Briefly centrifuge small vials before opening. Read entire protocol before performing the assay.SIRT1 Assay Buffer: Store at 4°C or -20°C. Warm to 37°C and add DTT to final concentration of 2 mM just before use. Make fresh as needed.1 M DTT: Store at -20°C. Thaw and keep on ice while in use. Use within two months.Substrate: Aliquot and Store at -20°C. Avoid repeated freeze/thaw. Use fresh tip each time. Use within two months.NAD: Reconstitute with 220 µl deionized water. Aliquot and store at -80°C. Avoid repeated freeze/thaw. Before use, dilute NAD by adding 2 µl of NAD stock solution to 58 µl of SIRT1 Assay Buffer without DTT. Make as much as needed. Use within two months.SIRT1 Enzyme: Thaw and mix gently by pipetting. Aliquot and store at -20°C. Use within two months.Inhibitor (Nicotinamide): Store at -20°C. Keep on ice while in use.Developer: Aliquot and store at -20°C. Avoid repeated freeze/thaw. Keep on ice while in use