Transglutaminase Inhibitor Screening Assay Kit
Cat.No. : | Kit-2277 |
Product Overview : | Transglutaminases (TGs) are enzymes that catalyze post-translational modifications of glutamine residues in proteins, via the formation of isopeptide bonds, esterification and deamination. The reaction produces insoluble macromolecular complexes and protein aggregates. Within the TG family of enzyme, tissue-specific Transglutaminase-2 (TG2) expression is proven to be a clinical target for multiple types of cancer and neurodegenerative diseases, including Alzheimer’s and Huntington’s diseases.Inhibition of TG2, with a Cystamine-derived drug candidate (currently in phase-II clinical trial for Huntington’s disease), has shown to have neuroprotective effects in models of neurodegeneration. Further, TG2 interacts with retinoblastoma protein (Rb), GTPase, and protein kinases through serine/threonine phosphorylation in cancer. Therefore, TG2 is a clinical validated candidate in these pathological diseases, and is a highly relevant therapeutic biomarker. Transglutaminase Inhibitor Screening Assay Kit utilizes the catalytic activity of TG2, in a deamidation reaction between donor and acceptor substrates, to generate a hydroxamate product. The stop solution reacts with the hydroxamate product, forming a purple complex that can be measured at 525 nm.In the presence of TG2 inhibitor, the enzyme loses its ability to catalyze the deamidation reaction, leading to a decrease in color development and a lower absorbance OD reading. This assay kit is simple and can be used to identify and characterize TG2 inhibitors in a high-throughput format. |
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- Gene Information
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Applications : | Screening/studying/characterizing Transglutaminase inhibitors. |
Storage : | -20°C |
Size : | 100 assays |
Products Types
◆ Recombinant Protein | ||
Tg-2125R | Recombinant Rat Tg Protein, His-tagged | +Inquiry |
TG-0056H | Recombinant Human TG Protein | +Inquiry |
TG-708H | Recombinant Human TG Protein, MYC/DDK-tagged | +Inquiry |
Tg-6386M | Recombinant Mouse Tg Protein, Myc/DDK-tagged | +Inquiry |
TG-2515H | Recombinant Human TG protein(1511-1610 aa), C-His-tagged | +Inquiry |
◆ Native Protein | ||
TG-8265H | Native Human Thyroids Thyroglobulin | +Inquiry |
TG-31519TH | Native Human TG | +Inquiry |
TG-393H | Native Human Thyroglobulin | +Inquiry |
◆ Assay kits | ||
Kit-0814 | Transglutaminase Activity Colorimetric Assay Kit | +Inquiry |
Kit-2036 | Triglyceride Colorimetric Assay Kit | +Inquiry |
Kit-0815 | Triglyceride Quantification Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Ask a questionCustomer Reviews (3)
Write a reviewEnhancing efficiency, the biomolecular reagent yields fast experimental results.
Suitable for long-term storage, the protein substance exhibits long-lasting stability.
Emphasizing product innovation, the manufacturer regularly introduces new protein reagents.
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