Acitve Recombinant Marmoset IL12B protein(Met1-Asn328), hFc-tagged
Cat.No. : | IL12B-1381M |
Product Overview : | Recombinant Marmoset IL12B (F7IQ84) (Met1-Asn328) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus. |
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Source : | HEK293 |
Species : | Marmoset |
Tag : | Fc |
Protein length : | Met1-Asn328 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Measured by its binding ability in a functional ELISA. 2.Immobilized marmoset IL12B-Fc at 10 μg/mL (100 μL/well) can bind biotinylated Human IL12RB1-His. The EC50 of Human IL12RB1-His is 0.66-1.53 μg/mL. |
Molecular Mass : | The recombinant marmoset IL12B is a disulfide-linked homodimer. The reduced monomer comprises 547 amino acids and has a calculated molecular mass of 61.9 KDa.The apparent molecular mass of the protein is approximately 66 KDa in SDS-PAGE. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Official Symbol : | IL12B |
Official Symbol : | IL12B |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewGood for cytokine assays.
Effective in Th2 cell differentiation.
High yield in expression.
Q&As (10)
Ask a questionChIP-seq identifies regulatory elements controlling IL12B gene expression.
Reporter assays monitor IL12B promoter activity under varying conditions.
Primary cell stimulation assays gauge IL12B response to pathogens.
siRNA knockdown uncovers IL12B's role in immune cell activation pathways.
Recombinant protein expression enables functional IL12B studies in vitro.
Co-IP validates IL12B's binding to specific signaling partners.
Genetic variant analysis reveals impacts on IL12B protein function.
Flow cytometry profiles IL12B expression across diverse immune cell types.
Cytokine secretion assays measure IL12B's response kinetics to stimuli.
Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.
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