Acitve Recombinant Rat ALCAM protein(Met1-Lys527), hFc-tagged

• Cat.No.: ALCAM-1355R
• Product Overview: Recombinant Rat ALCAM (O35112) (Met1-Lys527) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-hFc
• Protein length: Met1-Lys527
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Rat CD6-His at 10 μg/ml (100 μl/well) can bind Rat ALCAM-Fc3, The EC50 of Rat ALCAM-Fc3 is 19.5-45.5 ng/ml.
• Molecular Mass: The recombinant Rat ALCAM/Fc is a disulfide-linked homodimer. The reduced monomer comprises 741 amino acids and has a predicted molecular mass of 83.1 kDa. The apparent molecular mass of the protein is approximately 94 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Alcam activated leukocyte cell adhesion molecule [ Rattus norvegicus ]
• Official Symbol: ALCAM
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; CD166 antigen; HB2; KG-CAM; SB-10 antigen
• Gene ID: 79559
• mRNA Refseq: NM_031753
• Protein Refseq: NP_113941