Active Biotinylated Recombinant Human MICA Protein (Ala23-Gln308), Fc/Avi tagged

• Cat.No.: MICA-706H
• Product Overview: Biotinylated recombinant Human MICA protein (Ala23-Gln308) with Human IgG1 (Pro100-Lys330) Fc Avi-tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants.
• Source: CHO
• Species: Human
• Form: Disulfide-linked homodimer, biotinylated via Avi-tag
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D Fc Chimera is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human MICA Fc Chimera Avi-tag that produces 50% of the optimal binding response is found to be approximately 7.5-45 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 61 kDa
  SDS-PAGE: 85-100 kDa, under reducing conditions
• N-terminal Sequence Analysis: Ala23
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: MICA MHC class I polypeptide-related sequence A [ Homo sapiens (human) ]
• Official Symbol: MICA
• Synonyms: MICA; MHC class I polypeptide-related sequence A; PERB11.1; HLA class I antigen; stress inducible class I homolog; MHC class I chain-related protein A; MIC-A; FLJ36918; FLJ60820; MGC21250; MGC111087
• Gene ID: 100507436
• mRNA Refseq: NM_001177519
• Protein Refseq: NP_001170990
• MIM: 600169
• UniProt ID: Q29983