Active Recombinant Human MICA Protein, His-tagged

• Cat.No.: MICA-03H
• Product Overview: Recombinant human MICA, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Measured by its binding ability in a functional ELISA with Human NKG2D.
• Molecular Mass: 32.7 kDa (283aa) confirmed by MALDI-TOF
• AA Sequence: MEPHSLRYNLTVLSWDGSVQSGFLT EVHLDGQPFLRCDRQKCRAKPQGQW AEDVLGNKTWDRETRDLTGNGKDLR MTLAHIKDQKEGLHSLQEIRVCEIH EDNSTRSSQHFYYDGELFLSQNLET EEWTMPQSSRAQTLAMNVRNFLKED AMKTKTHYHAMHADCLQELRRYLKS GVVLRRTVPPMVNVTRSEASEGNIT VTCRASGFYPWNITLSWRQDGVSLS HDTQQWGDVLPDGNGTYQTWVATRI CQGEEQRFTCYMEHSGNHSTHPVPS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol

Gene Information

• Gene Name: MICA MHC class I polypeptide-related sequence A [ Homo sapiens (human) ]
• Official Symbol: MICA
• Synonyms: MICA; MHC class I polypeptide-related sequence A; MIC-A; PERB11.1; MHC class I polypeptide-related sequence A; HLA class I antigen; MHC class I chain-related protein A; MHC class I related chain A; MHC class I related sequence A; major histocompatibility complex class I chain-related protein A; stress inducible class I homolog
• Gene ID: 100507436
• mRNA Refseq: NM_000247
• Protein Refseq: NP_000238
• MIM: 600169
• UniProt ID: Q29983