Active GMP Recombinant Human CA9 protein

• Cat.No.: CA9-804HG
• Product Overview: GMP Recombinant Human CA9 protein(NP_001183.2)(376 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Source: HEK293
• Species: Human
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: The specific activity is >200 pmol/min/ug, and 4-nitrophenylacetate is used for detection (routine test). One unit refers to the amount of enzyme that hydrolyzes 1.0 p mole of 4-nitrophenylacetic acid salt into 4-nitrophenol at pH 7.5 per minute at room temperature
• Molecular Mass: Recombinant Human CA9 contains 376 aa. It has a predicted MW of 42.6 kDa.
• Protein length: DNA sequence encoding Human Carbonic Anhydrase IX(NP_001207.2) was expressed with His tag at the C-terminal.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CA9 carbonic anhydrase IX [ Homo sapiens ]
• Official Symbol: CA9
• Synonyms: CAIX; CA9; CA-IX; G250; MN; P54/58N; pMW1
• Gene ID: 768
• mRNA Refseq: NM_001216
• Protein Refseq: NP_001207
• MIM: 603179
• UniProt ID: Q16790

Reviews

08/04/2022

Exceptional consistency, vital for our research's reliability.

12/16/2021

Critical support for our protein analysis and quantification.

02/07/2021

Unmatched accuracy in protein identification and sequencing.

Q&As

What signaling pathways are known to regulate CA9 expression?

The HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.

How does CA9 contribute to pH regulation within the tumor microenvironment?

CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.

How is CA9 expression regulated in hypoxic conditions?

CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.

How are elevated levels of CA9 associated with cancer prognosis?

Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.

What post-translational modifications have been identified on CA9?

Potential modifications like glycosylation can be identified using techniques like mass spectrometry.

Are there specific inhibitors that target CA9, especially in cancer therapeutics?

Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.

What roles does CA9 play in tumor physiology?

CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.