Recombinant Human CA9 Protein, Fc-tagged, Alexa Fluor 555 conjugated
Cat.No. : | CA9-890HAF555 |
Product Overview : | Alexa Fluor 555 conjugated recombinant human CA9 precursor (NP_001207.2) (Met 1-Asp 414), fused with the Fc region of human IgG1 at the C-terminus, was produced in Human Cell. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Form : | Lyophilized |
Molecular Mass : | The recombinant human CA9/Fc is a disulfide-linked homodimer. The reduced monomer consists of 615 amino acids and predicts a molecular mass of 67.7 kDa. As a result of glycosylation, the apparent molecular mass of rh CA9/Fc monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions. |
Protein length : | 615 |
Endotoxin : | < 1.0 EU/ μg of the protein as determined by the LAL method. |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. |
Stability : | Samples are stable for up to 12 months from date of receipt at -70 centigrade. |
Storage : | Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Storage Buffer : | Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5 |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | CA9 carbonic anhydrase IX [ Homo sapiens ] |
Official Symbol : | CA9 |
Gene ID : | 768 |
mRNA Refseq : | NM_001216 |
Protein Refseq : | NP_001207 |
MIM : | 603179 |
UniProt ID : | Q16790 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionThe HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.
CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.
CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.
Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.
Potential modifications like glycosylation can be identified using techniques like mass spectrometry.
Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.
CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.
Customer Reviews (3)
Write a reviewExceptional consistency, vital for our research's reliability.
Critical support for our protein analysis and quantification.
Unmatched accuracy in protein identification and sequencing.
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