Active GMP Recombinant Human IL18 Protein

• Cat.No.: IL18-01HG
• Product Overview: Recombinant human IL18 protein, was expressed in CHO-K1 cell-derived in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: CHO
• Description: The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
• Form: Lyophilized powder
• Endotoxin: <20 EU/mg
• Bio-activity: >5.0 x 10^4 IU/mg
• Purity: >95.0% by high performance liquid chromatography (SEC-HPLC) and SDS-PAGE.
• Storage Buffer: 10mM Phosphate-Buffered Saline (pH 7.2)
• Storage: Stable storage at 4℃ for 7-10 days; for long-term storage, please store at -20℃ after dispensing.
• Sterility: Sterility
• Instructions: It is recommended that lyophilized GMP rhIL18 be dissolved in water for injection or sterilized ultrapure water at a concentration of not less than 100 μg/ml, pending further dilution to working concentration.

Gene Information

• Gene Name: IL18 interleukin 18 [ Homo sapiens (human) ]
• Official Symbol: IL18
• Synonyms: IGIF; IL-18; IL-1g; IL1F4
• Gene ID: 3606
• mRNA Refseq: NM_001243211.2
• Protein Refseq: NP_001230140.1
• UniProt ID: Q14116
• MIM: 600953

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193; Endotoxin: <20 EU/mg; Bio-activity: >5.0 x 10^4 IU/mg; QC: Activity test, SDS-PAGE, LAL, SEC-HPLC.