| Species : |
Human |
| Source : |
E. coli |
| Tag : |
Non |
| Form : |
Lyophilized from a 0.2 µm filtered concentrated solution in 25 mM Tris-HCl, 3 mM β-Me and 5% Trehalose, pH 8.0. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/mL, corresponding to a specific activity of > 1.0 × 10^9 U/mg. |
| Molecular Mass : |
Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids. |
| AASequence : |
SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA |
| Endotoxin : |
Less than 0.01 EU/μg of rHuIL-1α GMP as determined by LAL method. |
| Purity : |
> 98 % by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
