Active GMP Recombinant Porcine IL1A Protein, His-Tagged
|Product Overview :||GMP Recombinant Porcine IL1A Protein, His-Tagged was expressed in Escherichia coli cell-derived in an animal component free process under cGMP guidelines.|
- Gene Information
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|Description :||Interleukin 1 alpha (IL-1伪) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL-1伪 is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha.|
|Source :||Escherichia coli|
|Bio-activity :||Measure by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is <50 pg/mL.|
|AA Sequence :||MSATYSFQSNMKYNFMRVINHQCIL NDARNQSIIRDPSGQYLMAAVLNNL DEAVKFDMAAYTSNDDSQLPVTLRI SETRLFVSAQNEDEPVLLKELPETP KTIKDETSLLFFWEKHGNMDYFKSA AHPKLFIATRQEKLVHMAPGLPSVT DFQILENQS with polyhistidine tag at the C-terminus|
|Endotoxin :||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity :||>98% as determined by SDS-PAGE. Ni-NTA chromatography|
|Notes :||Please use within one month after protein reconstitution.|
|Storage :||Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.|
|Storage Buffer :||The protein was lyophilized from a solution containing 1X PBS, pH 7.4.|
|Reconstitution :||It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.|
|Gene Name :||IL1A interleukin 1 alpha [ Sus scrofa (pig) ]|
|Official Symbol :||IL1A|
|Gene ID :||397094|
|mRNA Refseq :||NM_214029.1|
|Protein Refseq :||NP_999194.1|
|UniProt ID :||P18430|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
Customer Reviews (3)Write a review
High solubility. -
Good for intracellular staining. -
Effective in apoptosis assays. -
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