Active Native Rabbit Glyceraldehyde-3-phosphate Dehydrogenase

• Cat.No.: GAPDH-126R
• Product Overview: Native Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) was purified from rabbit muscle.

Specification

• Description: Glyceraldehyde-3-phosphatedehydrogenase (GAPDH; EC 1.2.1.12) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamideadenine dinucleotide (NAD). The enzyme is present in such widely separated forms as man, lobster, and E. coli. Its rate of evolutionary change is one of the slowest known. Recent evidence demonstrates that mammalian GAPDH displays a number of diverse activities unrelated to its glycolytic function. These include its role in membrane fusion, microtubule bundling, phosphotransferaseactivity, nuclear RNA export, DNA replication and DNA repair. These new activities may be related to the subcellular localization and oligomeric structure of GAPDH in vivo. Furthermore, other investigations suggest that GAPDH is involved in apoptosis, age-relatedneurodegenerative disease, prostate cancer and viral pathogenesis. Intriguingly, GAPDH is also a unique target of nitric oxide. Recently GAPDH has been shown to be a common target of a number of compounds with potent antiproliferative activities and thus a potential target for chemotherapeutic intervention.
• Source: Rabbit
• Species: Rabbit
• Form: Lyophilized powder form.
• Bio-activity: 1mg (80 Units)
• Unit Definition: One unit will reduce 1 micromole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per minute with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 oC.
• Storage: -20°C in aliquots. Freeze-thaw cycles must be avoided once the AC2tock aliquot is diluted.
• Tag: Non

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.