Recombinant Full Length Human GAPDH Protein, C-Flag-tagged

• Cat.No.: GAPDH-15HFL
• Product Overview: Recombinant Full Length Human GAPDH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 35.9 kDa
• AA Sequence: MGKVKVGVNGFGRIGRLVTRAAFNS GKVDIVAINDPFIDLNYMVYMFQYD STHGKFHGTVKAENGKLVIN GNPITIFQERDPSKIKWGDAGAEYV VESTGVFTTMEKAGAHLQGGAKRVI ISAPSADAPMFVMGVNHEKY DNSLKIISNASCTTNCLAPLAKVIH DNFGIVEGLMTTVHAITATQKTVDG PSGKLWRDGRGALQNIIPAS TGAAKAVGKVIPELNGKLTGMAFRV PTANVSVVDLTCRLEKPAKYDDIKK VVKQASEGPLKGILGYTEHQ
  VVSSDFNSDTHSSTFDAGAGIALND HFVKLISWYDNEFGYSNRVVDLMAH MASKETRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways

Gene Information

• Gene Name: GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: GAPDH
• Synonyms: G3PD; GAPD; HEL-S-162eP
• Gene ID: 2597
• mRNA Refseq: NM_002046.7
• Protein Refseq: NP_002037.2
• MIM: 138400
• UniProt ID: P04406