Active Recombinant Bovine NOS3 Protein

• Cat.No.: NOS3-22B
• Product Overview: Active recombinant bovine N-terminal His-tagged eNOS expressed in E. coli

Specification

• Species: Bovine
• Source: E.coli
• Tag: His
• Protein Length: 2-1205 aa
• Description: Produces nitric oxide (NO) which is a messenger molecule with diverse functions.
• Form: 50 mM HEPES, pH 7.8, with 0.5 mM DTT, 0.5 mM EDTA, 300 mM sodium chloride, 2 mM calcium chloride, 10 μM tetrahydro-L-biopterin (hydrochloride), and 20% glycerol
• Molecular Mass: 134 kDa
• Storage: At -80 centigrade. Stability: ≥ 1 year
• Shipping: Dry ice in continental US; may vary elsewhere
• Reference: 1. Qian, J., and Fulton, D. Post-translational regulation of endothelial nitric oxide synthase in vascular endothelium. Front. Physiol. 4, 347 (2013). 2. Heiss, C., Rodriguez-Mateos, A., and Kelm, M. Central role of eNOS in the maintenance of endothelial homeostasis. Antioxid. Redox Signal. 22(14), 1230-1242 (2015). 3. Siragusa, M., and Fleming, I. The eNOS signalosome and its link to endothelial dysfunction. Pflugers Arch. 468(7), 1125-1137 (2016).
• Unit Definition: One unit of enzyme produces 1 nmol of nitric oxide per minute at 37 centigrade in 50 mM HEPES, pH 7.8, with 5 μM oxyhemoglobin, 1 mM calcium chloride, 20 μg/mL calmodulin, 0.1 mM NADPH, 50 M arginine, 12 μM tetrahydrobiopterin, and 170 μM DTT.

Gene Information

• Gene Name: NOS3 nitric oxide synthase 3 [ Bos taurus (domestic cattle) ]
• Official Symbol: NOS3
• Synonyms: NOS3; nitric oxide synthase 3; nitric oxide synthase 3; EC-NOS; NOS type III; NOSIII; cNOS; constitutive NOS; eNOS; endothelial NOS; endothelial nitric oxide synthase; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase, endothelial; EC 1.14.13.39
• Gene ID: 287024
• mRNA Refseq: NM_181037
• Protein Refseq: NP_851380
• UniProt ID: F1MY54