Active Recombinant Canine ANGPT2 protein(Lys274-Phe495), His-tagged

• Cat.No.: ANGPT2-1040C
• Product Overview: Recombinant Canine ANGPT2 (NP_001041591.1) (Lys274-Phe495) was expressed in HEK293 with a polyhistidine tag at the N-terminus.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: Lys274-Phe495
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. 2. Immobilized Canine ANGPT2 at 10μg/mL (100μL/well) can bind human Tie2-Fch, the EC50 of human Tie2-Fch is 10-50 ng/mL.
• Molecular Mass: The recombinant Canine ANGPT2 consists 241 amino acids and predicts a molecular mass of 27.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.