| Species : |
Canine |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
77-233 aa |
| Description : |
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated. |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag with a linear range of 0.3-1 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU/μg by the LAL method/rFC method. |
| Purity : |
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
