Cat.No. : |
TNF-504C
Optional Service: Optional requirements on this protein
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Product Overview : |
Recombinant Canine TNF (Accession # CAA64403) Val77-Leu233, fused with an N-terminal Met, was produced in E. coli-derived. |
Source : |
E. coli |
Species : |
Canine |
Predicted N Terminal : |
Met |
Form : |
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. |
Bio-activity : |
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 0.6-3 ng/mL. |
Molecular Mass : |
Recombinant Canine TNF, Met-tagged has a calculated MW of 17.3 kDa. |
Purity : |
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : |
Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |