| Cat.No. : |
TNF-504C
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Canine TNF (Accession # CAA64403) Val77-Leu233, fused with an N-terminal Met, was produced in E. coli-derived. |
| Source : |
E. coli |
| Species : |
Canine |
| Predicted N Terminal : |
Met |
| Form : |
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. |
| Bio-activity : |
Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 0.6-3 ng/mL. |
| Molecular Mass : |
Recombinant Canine TNF, Met-tagged has a calculated MW of 17.3 kDa. |
| Purity : |
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
| Reconstitution : |
Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
