Active Recombinant Cynomolgus IL18BP protein, His-tagged, Biotinylated

• Cat.No.: IL18BP-57CB
• Product Overview: Biotinylated Recombinant Cynomolgus IL18BP protein(A0A2K5UDJ4)(Thr28-Pro207) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• Protein Length: 28-207 a.a.
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Biotinylated Cynomolgus IL-18BP, His tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 1.67 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 21.14 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
• Proteinlength: Thr28-Pro207
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 90% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: IL18BP interleukin 18 binding protein [ Homo sapiens ]
• Official Symbol: IL18BP
• Synonyms: IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; IL18BPa; MC51L 53L 54L homolog gene product; IL-18BP; tadekinig-alfa; MC51L-53L-54L homolog gene product
• Gene ID: 10068
• mRNA Refseq: NM_001039659
• Protein Refseq: NP_001034748
• MIM: 604113
• UniProt ID: O95998