Source : |
HEK293 |
Species : |
Cynomolgus |
Tag : |
Fc |
Form : |
Lyophilized from 0.22 μm filtered solution in 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-4, Fc Tag with a linear range of 0.1-4 ng/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-50 kDa and 53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Proteinlength : |
His25-Ser153 |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>90% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Official Symbol : |
IL4 |