Cat.No. : |
TNF-1168C
Optional Service: Optional requirements on this protein
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Product Overview : |
Recombinant cynomolgus TNF [(Identical to the rhesus TNF (NP_001040614.1)] (Val77-Leu233) was expressed and purified with an initial Met. |
Source : |
E. coli |
Species : |
Cynomolgus |
Predicted N Terminal : |
Met |
Form : |
Lyophilized from sterile 50 mM Tris, 50 mM NaCl, pH 8.0, 5%~8% trehalose and mannitol. |
Bio-activity : |
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 50-300 pg/ml. |
Molecular Mass : |
The recombinant cynomolgus TNF consists of 158 amino acids and has a calculated molecular mass of 17.4 kDa. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : |
>90 % as determined by SDS-PAGE. |
Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : |
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |