| Cat.No. : |
TNF-1168C
Optional Service: Optional requirements on this protein
|
| Product Overview : |
Recombinant cynomolgus TNF [(Identical to the rhesus TNF (NP_001040614.1)] (Val77-Leu233) was expressed and purified with an initial Met. |
| Source : |
E. coli |
| Species : |
Cynomolgus |
| Predicted N Terminal : |
Met |
| Form : |
Lyophilized from sterile 50 mM Tris, 50 mM NaCl, pH 8.0, 5%~8% trehalose and mannitol. |
| Bio-activity : |
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 50-300 pg/ml. |
| Molecular Mass : |
The recombinant cynomolgus TNF consists of 158 amino acids and has a calculated molecular mass of 17.4 kDa. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
>90 % as determined by SDS-PAGE. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
