Cat. No. : |
Tnf-310M |
Description : |
Tumor necrosis factor (TNF, cachexin or cachectin formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. |
Species : |
Mouse |
Source : |
Streptomyces Lividans |
Form : |
Each mg contains 50mM Tris-HCL pH-8 and 20mM NaCL. |
Purity : |
Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Bio-activity : |
rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108IU/mg. |
Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
Solubility : |
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions. |
Purity Greater than 98% as determined by : |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
Protein Content determined by : |
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
Amino acid sequence : |
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
Pathways : |
Adipocytokine signaling pathway; Adipogenesis; African trypanosomiasis; Allograft rejection; Alzheimer"s disease; Amoebiasis; Antigen processing and presentation; Apoptosis; Asthma; Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response (BioCarta); Death Receptor Signalling; Diabetes pathways; Dilated cardiomyopathy; Extrinsic Pathway for Apoptosis; Fc epsilon RI signaling pathway; Tuberculosis; Type I diabetes mellitus; Type II diabetes mellitus |