Active Recombinant Mouse Tumor Necrosis Factor Tnf-310M

Active Recombinant Mouse Tumor Necrosis Factor


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Cat.No. : Tnf-310M Optional Service: Optional requirements on this protein
Product Overview : Recombinant murine TNF-alpha produced in S.Lividians is a single,non-glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32 Dalton.
Description : Tumor necrosis factor (TNF, cachexin or cachectin formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction.
Species : Mouse
Source : Streptomyces Lividans
Form : Each mg contains 50mM Tris-HCL pH-8 and 20mM NaCL.
Purity : Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108IU/mg.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 98% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Adipocytokine signaling pathway; Adipogenesis; African trypanosomiasis; Allograft rejection; Alzheimer"s disease; Amoebiasis; Antigen processing and presentation; Apoptosis; Asthma; Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response (BioCarta); Death Receptor Signalling; Diabetes pathways; Dilated cardiomyopathy; Extrinsic Pathway for Apoptosis; Fc epsilon RI signaling pathway; Tuberculosis; Type I diabetes mellitus; Type II diabetes mellitus
PDBrendering based on 1TNF. :

Gene Name : Tnf tumor necrosis factor [ Mus musculus ]
Official Symbol : Tnf
Synonyms : Tnf; tumor necrosis factor; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; MGC151434; TNF-alpha; TNF-a; TNF alpha; cachectin; tumor necrosis factor alpha;tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; APC1 protein; cachectin; TNF superfamily, member 2; TNFA; TNF, macrophage-derived; TNF, monocyte-derived; tumor necrosis factor-alpha; tumor necrosis factor (TNF superfamily, member 2); Cachectin
Gene ID : 21926
mRNA Refseq : NM_013693
Protein Refseq : NP_038721
UniProt ID : P06804
Chromosome Location : 17 B1; 17 19.06 cM
Function : cytokine activity; identical protein binding; protease binding; protein binding; transcription regulatory region DNA binding; tumor necrosis factor receptor binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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