| Species : |
Dog |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
103-260 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a member of the interleukin 1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1β can induced IL-8 production in A549 cells. To test the bioactivity of recombinant dog IL-1β, A549 cells were seeded into 24-well plate at a density of 1x10^5 cells/mL, and allowed to attach overnight before treated with certain concentrations of recombinant dog IL-1β for 48h and IL-8 levels in the cell supernatant were determined by ELISA (SEA080Hu). IL-8 levels in the cell supernatant of A549 cells increased significantly after stimulated with IL-1β, the EC50 was 56 ug/ml. |
| Molecular Mass : |
19.3kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 97% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
