Active Recombinant Cynomolgus IL1B Protein

• Cat.No.: IL1B-1167C
• Product Overview: Recombinant mature form of Cynomolgus (Macaca fascicularis) IL1B (Ala 117-Ser 269) was expressed and purified, with an initial Met.

Specification

• Species: Cynomolgus
• Source: E.coli
• Tag: Non
• Protein Length: 117-269 a.a.
• Predicted N Terminal: Met 1
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 3-15 pg/mL.
• Molecular Mass: The recombinant Cynomolgus IL1B consists of 154 amino acids and has a calculated molecular mass of 17.5 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IL1B interleukin 1 beta [ Macaca fascicularis ]
• Official Symbol: IL1B
• Synonyms: IL-1 beta; Interleukin-1 beta; interleukin 1, beta
• Gene ID: 102119749
• Chromosome Location: chromosome: 13
• Pathway: AGE-RAGE signaling pathway in diabetic complications, organism-specific biosystem; Alzheimer"s disease, conserved biosystem; Amoebiasis, conserved biosystem