Active Recombinant Feline IL10
Cat.No. : | IL10-215F |
Product Overview : | A DNA sequence encoding the feline IL10 (P55029.2) (Ser19-Ile178) was expressed with an initial Met at the N-terminal. |
- Specification
- Gene Information
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Source : | E. coli |
Species : | Feline |
Form : | Lyophilized from sterile PBS, pH7.4. |
Bio-activity : | 1. Immobilized felineIL10at 10 μg/ml (100 μl/well) can bind Cynomolgus IL10RA-Fc, The EC50 of Cynomolgus IL10RA-Fc is 0.14-0.32 μg/ml.2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 0.1-0.6 n |
Molecular Mass : | The recombinant feline IL10 consists of 161 amino acids and has a predicted molecular mass of 18.9 kDa. The apparent molecular mass of it is approximately 19 kDa in SDS-PAGE under reducing conditions. |
Protein length : | Ser19-Ile178 |
Endotoxin : | < 1.0 eu per μg of the protein as determined by the LAL method. |
Purity : | >95 % as determined by SDS-PAGE |
Stability : | Samples are stable for up to twelve months from date of receipt at -70℃ |
Storage : | Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | Hardcopy of COA with reconstitution instruction is sent along with the products. |
Gene Name : | IL10?interleukin 10 [?Felis catus?(domestic cat) ] |
Official Symbol : | IL10 |
Synonyms : | IL10; interleukin 10; CSIF; IL-10; cytokine synthesis inhibitory factor |
Gene ID : | 493683 |
mRNA Refseq : | NM_001009209 |
Protein Refseq : | NP_001009209 |
UniProt ID : | P55029 |
Chromosome Location : | F1 |
Pathway : | African trypanosomiasis; Allograft rejection; Autoimmune thyroid disease |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.
Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.
Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.
Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.
In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.
State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.
Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.
Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.
Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.
CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.
Customer Reviews (3)
Write a reviewEffective in anti-inflammatory assays.
High purity levels.
Stable under long-term storage.
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