Active Recombinant Firefly Luciferase Protein (1-550), N-His-tagged

• Cat.No.: Luciferase-787F
• Product Overview: Recombinant firefly Luciferase (1-550), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening.
• Source: E. coli
• Species: Firefly
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is 1×10^10 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at 25 centigrade.
• Molecular Mass: 63 kDa (571aa) confirmed by MALDI-TOF
• Protein length: 1-550
• AA Sequence: MEDAKNIKKGPAPFYPLEDGTAGEQ LHKAMKRYALVPGTIAFTDAHIEVN ITYAEYFEMSVRLAEAMKRYGLNTN HRIVVCSENSLQFFMPVLGALFIGV AVAPANDIYNERELLNSMNISQPTV VFVSKKGLQKILNVQKKLPIIQKII IMDSKTDYQGFQSMYTFVTSHLPPG FNEYDFVPESFDRDKTIALIMNSSG STGLPKGVALPHRTACVRFSHARDP IFGNQIIPDTAILSVVPFHHGFGMF TTLGYLICGFRVVLMYRFEEELFLR SLQDYKIQSALLVPTLFSFFAKSTL IDKYDLSNLHEIASGGAPLSKEVGE AVAKRFHLPGIRQGYGLTETTSAIL ITPEGDDKPGAVGKVVPFFEAKVVD LDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDLAY WDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDD DAGELPAAVVVLEHGKTMTEKEIVD YVASQVTTAKKLRGGVVFVDEVPKG LTGKLDARKIREILIKAKKGGKSKL
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Official Symbol: Luciferase
• Synonyms: Luciferase; Luciferin 4-monooxygenase, LuC
• UniProt ID: A3KBZ5

Gene Information

• Official Symbol: Luciferase
• Synonyms: Luciferase; Luciferin 4-monooxygenase, LuC
• UniProt ID: A3KBZ5

Citation

Cry Protein Crystals: A Novel Platform for Protein Delivery

Journal: PLoS ONE    PubMed ID: 26030844    Data: 2015/6/1

Authors: Manoj S. Nair, Marianne M. Lee, Raffi V. Aroian

Article Snippet:The protein concentrations of Cry3Aa-luciferase crystal and recombinant Firefly luciferase protein (Creative BioMart) were determined using the Bradford protein assay (BioRad).. Measurement of the in vivo luminescence was performed on an IVIS 100 system (Xenogen).Measurement of the in vivo luminescence was performed on an IVIS 100 system (Xenogen).

Publication :

The effect of spin exchange interaction on protein structural stability (2022)

Reviews

05/18/2022

Worked well, happy customer.

08/30/2021

Great in lab experiments.

09/04/2020

Satisfied with the purchase.

Q&As

How is luciferase utilized in bioluminescence imaging?

It allows for real-time tracking of cellular and molecular processes in living organisms.

What is the primary function of luciferase in organisms that produce it?

Luciferase enables bioluminescence, helping organisms attract mates, prey, or deter predators.

How does luciferase produce light in a biochemical reaction?

It catalyzes the oxidation of luciferin, releasing light.

In which organisms is luciferase commonly found?

Luciferase is found in fireflies, certain jellyfish, and some fungi and bacteria.

Are there different types of luciferase, and how do they vary?

Yes, there are different types, varying in their substrate specificity and the color of emitted light.

What is the role of ATP in the luciferase reaction?

ATP provides energy for the luciferase reaction, converting luciferin to oxyluciferin.

What advantages does the use of luciferase offer in scientific research?

Luciferase provides a non-invasive and highly sensitive method for studying biological phenomena.