Active Recombinant Full Length Human BUB1B Protein, GST-tagged

• Cat.No.: BUB1B-4915HF
• Product Overview: Full-length human BUBR1 [1-1050(end) amino acids of accession number NP_001202.4] was expressed as N-terminal GST-fusion protein (147 kDa) using baculovirus expression system. GST-BUBR1 was purified by using glutathione sepharose chromatography.

Specification

• Source: Insect (sf21)
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG.
• Protein length: 1-1050(end)
• Purity: 74%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Official Symbol: BUB1B
• Synonyms: BUB1B;BUB1beta;BUBR1;Bub1A;MAD3L;MVA1;SSK1;hBUBR1
• Protein Refseq: NP_001202.4
• UniProt ID: O60566