Human BUB1B Knockdown Cell Lysate

• Cat.No.: BUB1B-076HKCL
• Product Overview: WB-validated BUB1B Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 120 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg BUB1B KD HeLa cell lysate
• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: BUB1B budding uninhibited by benzimidazoles 1 homolog beta (yeast) [ Homo sapiens (human) ]
• Official Symbol: BUB1B
• Synonyms: BUB1B; budding uninhibited; budding uninhibited by benzimidazoles 1 (yeast homolog), beta; mitotic checkpoint serine/threonine-protein kinase BUB1 beta; Bub1A; BUBR1; MAD3L; SSK1; mitotic checkpoint kinase MAD3L; MAD3/BUB1-related protein kinase; hBUBR1; BUB1beta
• Gene ID: 701
• mRNA Refseq: NM_001211
• Protein Refseq: NP_001202
• MIM: 602860
• UniProt ID: O60566