Active Recombinant Full Length Human DNMT1 Protein, C-Flag-tagged

• Cat.No.: DNMT1-201HFL
• Product Overview: Recombinant Full Length Human DNMT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: DNMT1 activity verified in a biochemical assay: DNMT1 (DNA (cytosine-5-)-methyltransferase 1) is a key methyltransferase that is responsible for maintaining methylation patterns established in development. DNMT1 preferentially methylates hemi-methylated CpG di-nucleotides and associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. Varying concentrations of DNMT1 were added to a microplate containing a bound methyltransferase substrate. After incubation, the resulting methylated DNA residues were detected immunologically and a colorimetric signal was generated and measured.
• Molecular Mass: 184.6 kDa
• AA Sequence: MPARTAPARVPTLAVPAISLPDDVR RRLKDLERDSLTEKECVKEKLNLLH EFLQTEIKNQLCDLETKLRK EELSEEGYLAKVKSLLNKDLSLENG AHAYNREVNGRLENGNQARSEARRV GMADANSPPKPLSKPRTPRR SKSDGEAKRSRDPPASASQVTGIRA EPSPSPRITRKSTRQTTITSHFAKG PAKRKPQEESERAKSDESIK EEDKDQDEKRRRVTSRERVARPLPA EEPERAKSGTRTEKEEERDEKEEKR LRSQTKEPTPKQKLKEEPDR EARAGVQADEDEDGDEKDEKKHRSQ PKDLAAKRRPEEKEPEKVNPQISDE KDEDEKEEKRRKTTPKEPTE KKMARAKTVMNSKTHPPKCIQCGQY LDDPDLKYGQHPPDAVDEPQMLTNE KLSIFDANESGFESYEALPQ HKLTCFSVYCKHGHLCPIDTGLIEK NIELFFSGSAKPIYDDDPSLEGGVN GKNLGPINEWWITGFDGGEK ALIGFSTSFAEYILMDPSPEYAPIF GLMQEKIYISKIVVEFLQSNSDSTY EDLINKIETTVPPSGLNLNR FTEDSLLRHAQFVVEQVESYDEAGD SDEQPIFLTPCMRDLIKLAGVTLGQ RRAQARRQTIRHSTREKDRG PTKATTTKLVYQIFDTFFAEQIEKD DREDKENAFKRRRCGVCEVCQQPEC GKCKACKDMVKFGGSGRSKQ ACQERRCPNMAMKEADDDEEVDDNI PEMPSPKKMHQGKKKKQNKNRISWV GEAVKTDGKKSYYKKVCIDA ETLEVGDCVSVIPDDSSKPLYLARV TALWEDSSNGQMFHAHWFCAGTDTV LGATSDPLELFLVDECEDMQ LSYIHSKVKVIYKAPSENWAMEGGM DPESLLEGDDGKTYFYQLWYDQDYA RFESPPKTQPTEDNKFKFCV SCARLAEMRQKEIPRVLEQLEDLDS RVLYYSATKNGILYRVGDGVYLPPE AFTFNIKLSSPVKRPRKEPV DEDLYPEHYRKYSDYIKGSNLDAPE PYRIGRIKEIFCPKKSNGRPNETDI KIRVNKFYRPENTHKSTPAS YHADINLLYWSDEEAVVDFKAVQGR CTVEYGEDLPECVQVYSMGGPNRFY FLEAYNAKSKSFEDPPNHAR SPGNKGKGKGKGKGKPKSQACEPSE PEIEIKLPKLRTLDVFSGCGGLSEG FHQAGISDTLWAIEMWDPAA QAFRLNNPGSTVFTEDCNILLKLVM AGETTNSRGQRLPQKGDVEMLCGGP PCQGFSGMNRFNSRTYSKFK NSLVVSFLSYCDYYRPRFFLLENVR NFVSFKRSMVLKLTLRCLVRMGYQC TFGVLQAGQYGVAQTRRRAI ILAAAPGEKLPLFPEPLHVFAPRAC QLSVVVDDKKFVSNITRLSSGPFRT ITVRDTMSDLPEVRNGASAL EISYNGEPQSWFQRQLRGAQYQPIL RDHICKDMSALVAARMRHIPLAPGS DWRDLPNIEVRLSDGTMARK LRYTHHDRKNGRSSSGALRGVCSCV EAGKACDPAARQFNTLIPWCLPHTG NRHNHWAGLYGRLEWDGFFS TTVTNPEPMGKQGRVLHPEQHRVVS VRECARSQGFPDTYRLFGNILDKHR QVGNAVPPPLAKAIGLEIKL
  CMLAKARESASAKIKEEEAAKDTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways

Gene Information

• Gene Name: DNMT1 DNA methyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: DNMT1
• Synonyms: AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
• Gene ID: 1786
• mRNA Refseq: NM_001130823.3
• Protein Refseq: NP_001124295.1
• MIM: 126375
• UniProt ID: P26358