Recombinant Full Length Human DNMT1 Protein, His&GST tagged

• Cat.No.: DNMT1-02H
• Product Overview: Full length recombinant DNMT1 protein (accession number NP_001370.1) was expressed in Sf9 cells and contains an N-terminal 6×His Tag and FLAG-Tag with a molecular weight of 188.2 kDa.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-1616 aa
• Description: DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally ass centigradeiated with transcriptional repression.
• Form: 25 mM HEPES NaOH pH 7.5, 500 mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5 mM TCEP.
• Molecular Mass: 188.2 kDa
• Purity: > 90% by SDS-PAGE.
• Applications: Recombinant DNMT1 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. DNMT Activity Assay Conditions: Assay was performed using DNMT Activity / Inhibition Assay, for 1 hour at 37 centigrade.
• Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 centigrade to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
• Concentration: 0.8 μg/μL

Gene Information

• Gene Name: DNMT1 DNA (cytosine-5-)-methyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: DNMT1
• Synonyms: DNMT1; DNA (cytosine-5-)-methyltransferase 1; DNMT; DNA (cytosine-5)-methyltransferase 1; CXXC9; MCMT; m.HsaI; DNA MTase HsaI; CXXC finger protein 9; DNA methyltransferase 1; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9; AIM; HSN1E; FLJ16293; MGC104992
• Gene ID: 1786
• mRNA Refseq: NM_001130823
• Protein Refseq: NP_001124295
• MIM: 126375
• UniProt ID: P26358