Active Recombinant Full Length Human EEF1A1 Protein, C-Flag-tagged

• Cat.No.: EEF1A1-43HFL
• Product Overview: Recombinant Full Length Human EEF1A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: ELISA capture for autoantibodies
  Biolayer interferometry (BLI) assay
  Binding assay
• Molecular Mass: 50 kDa
• AA Sequence: MGKEKTHINIVVIGHVDSGKSTTTG HLIYKCGGIDKRTIEKFEKEAAEMG KGSFKYAWVLDKLKAERERG ITIDISLWKFETSKYYVTIIDAPGH RDFIKNMITGTSQADCAVLIVAAGV GEFEAGISKNGQTREHALLA YTLGVKQLIVGVNKMDSTEPPYSQK RYEEIVKEVSTYIKKIGYNPDTVAF VPISGWNGDNMLEPSANMPW FKGWKVTRKDGNASGTTLLEALDCI LPPTRPTDKPLRLPLQDVYKIGGIG TVPVGRVETGVLKPGMVVTF APVNVTTEVKSVEMHHEALSEALPG DNVGFNVKNVSVKDVRRGNVAGDSK NDPPMEAAGFTAQVIILNHP GQISAGYAPVLDCHTAHIACKFAEL KEKIDRRSGKKLEDGPKFLKSGDAA IVDMVPGKPMCVESFSDYPP
  LGRFAVRDMRQTVAVGVIKAVDKKA AGAGKVTKSAQKAQKAKTRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: EEF1A1 eukaryotic translation elongation factor 1 alpha 1 [ Homo sapiens (human) ]
• Official Symbol: EEF1A1
• Synonyms: CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; EF1A1; LENG7; eEF1A-1; GRAF-1EF; EF1alpha1
• Gene ID: 1915
• mRNA Refseq: NM_001402.6
• Protein Refseq: NP_001393.1
• MIM: 130590
• UniProt ID: P68104