Active Recombinant Full Length Human ELAVL1 Protein, C-Flag-tagged

• Cat.No.: ELAVL1-75HFL
• Product Overview: Recombinant Full Length Human ELAVL1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA reaction positive control
• Molecular Mass: 35.9 kDa
• AA Sequence: MSNGYEDHMAEDCRGDIGRTNLIVN YLPQNMTQDELRSLFSSIGEVESAK LIRDKVAGHSLGYGFVNYVT AKDAERAINTLNGLRLQSKTIKVSY ARPSSEVIKDANLYISGLPRTMTQK DVEDMFSRFGRIINSRVLVD QTTGLSRGVAFIRFDKRSEAEEAIT SFNGHKPPGSSEPITVKFAANPNQN KNVALLSQLYHSPARRFGGP VHHQAQRFRFSPMGVDHMSGLSGVN VPGNASSGWCIFIYNLGQDADEGIL WQMFGPFGAVTNVKVIRDFN
  TNKCKGFGFVTMTNYEEAAMAIASL NGYRLGDKILQVSFKTNKSHKTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: ELAVL1 ELAV like RNA binding protein 1 [ Homo sapiens (human) ]
• Official Symbol: ELAVL1
• Synonyms: HUR; Hua; MelG; ELAV1
• Gene ID: 1994
• mRNA Refseq: NM_001419.3
• Protein Refseq: NP_001410.2
• MIM: 603466
• UniProt ID: Q15717