Active Recombinant Full Length Human ESR1 Protein, C-Flag-tagged

• Cat.No.: ESR1-38HFL
• Product Overview: Recombinant Full Length Human ESR1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: Binding assay
• Molecular Mass: 66 kDa
• AA Sequence: MTMTLHTKASGMALLHQIQGNELEP LNRPQLKIPLERPLGEVYLDSSKPA VYNYPEGAAYEFNAAAAANA QVYGQTGLPYGPGSEAAAFGSNGLG GFPPLNSVSPSPLMLLHPPPQLSPF LQPHGQQVPYYLENEPSGYT VREAGPPAFYRPNSDNRRQGGRERL ASTNDKGSMAMESAKETRYCAVCND YASGYHYGVWSCEGCKAFFK RSIQGHNDYMCPATNQCTIDKNRRK SCQACRLRKCYEVGMMKGGIRKDRR GGRMLKHKRQRDDGEGRGEV GSAGDMRAANLWPSPLMIKRSKKNS LALSLTADQMVSALLDAEPPILYSE YDPTRPFSEASMMGLLTNLA DRELVHMINWAKRVPGFVDLTLHDQ VHLLECAWLEILMIGLVWRSMEHPG KLLFAPNLLLDRNQGKCVEG MVEIFDMLLATSSRFRMMNLQGEEF VCLKSIILLNSGVYTFLSSTLKSLE EKDHIHRVLDKITDTLIHLM AKAGLTLQQQHQRLAQLLLILSHIR HMSNKGMEHLYSMKCKNVVPLYDLL LEMLDAHRLHAPTSRGGASV
  EETDQSHLATAGSTSSHSLQKYYIT GEAEGFPATVTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

Gene Information

• Gene Name: ESR1 estrogen receptor 1 [ Homo sapiens (human) ]
• Official Symbol: ESR1
• Synonyms: ER; ESR; Era; ESRA; ESTRR; NR3A1
• Gene ID: 2099
• mRNA Refseq: NM_000125.4
• Protein Refseq: NP_000116.2
• MIM: 133430
• UniProt ID: P03372