Active Recombinant Full Length Human EZR Protein, C-Flag-tagged

• Cat.No.: EZR-127HFL
• Product Overview: Recombinant Full Length Human EZR Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: ELISA capture for autoantibodies
• Molecular Mass: 69.2 kDa
• AA Sequence: MPKPINVRVTTMDAELEFAIQPNTT GKQLFDQVVKTIGLREVWYFGLHYV DNKGFPTWLKLDKKVSAQEV RKENPLQFKFRAKFYPEDVAEELIQ DITQKLFFLQVKEGILSDEIYCPPE TAVLLGSYAVQAKFGDYNKE VHKSGYLSSERLIPQRVMDQHKLTR DQWEDRIQVWHAEHRGMLKDNAMLE YLKIAQDLEMYGINYFEIKN KKGTDLWLGVDALGLNIYEKDDKLT PKIGFPWSEIRNISFNDKKFVIKPI DKKAPDFVFYAPRLRINKRI LQLCMGNHELYMRRRKPDTIEVQQM KAQAREEKHQKQLERQQLETEKKRR ETVEREKEQMMREKEELMLR LQDYEEKTKKAERELSEQIQRALQL EEERKRAQEEAERLEADRMAALRAK EELERQAVDQIKSQEQLAAE LAEYTAKIALLEEARRRKEDEVEEW QHRAKEAQDDLVKTKEELHLVMTAP PPPPPPVYEPVSYHVQESLQ DEGAEPTGYSAELSSEGIRDDRNEE KRITEAEKNERVQRQLLTLSSELSQ ARDENKRTHNDIIHNENMRQ
  GRDKYKTLRQIRQGNTKQRIDEFEA LTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton

Gene Information

• Gene Name: EZR ezrin [ Homo sapiens (human) ]
• Official Symbol: EZR
• Synonyms: CVL; CVIL; VIL2; HEL-S-105
• Gene ID: 7430
• mRNA Refseq: NM_003379.5
• Protein Refseq: NP_003370.2
• MIM: 123900
• UniProt ID: P15311