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Active Recombinant Full Length Human FYN Protein, C-Flag-tagged

Cat.No. : FYN-822HFL
Product Overview : Recombinant Full Length Human FYN Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.
  • Specification
  • Gene Information
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Description : This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
Source : Mammalian cells
Species : Human
Tag : Flag
Form : 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bio-activity : FYN activity verified in a biochemical assay: FYN (FYN oncogene related to SRC, FGR, YES) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FYN is a non-receptor tyrosine kinase and is a member of the Src kinase family consisting of Src, Fyn, Yes, Lck, Lyn, Hck, Fgr and Blk. Varying concentrations of FYN were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “&DeltaR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Molecular Mass : 60.6 kDa
AA Sequence : MGCVQCKDKEATKLTEERDGSLNQS SGYRYGTDPTPQHYPSFGVTSIPNY NNFHAAGGQGLTVFGGVNSS SHTGTLRTRGGTGVTLFVALYDYEA RTEDDLSFHKGEKFQILNSSEGDWW EARSLTTGETGYIPSNYVAP VDSIQAEEWYFGKLGRKDAERQLLS FGNPRGTFLIRESETTKGAYSLSIR DWDDMKGDHVKHYKIRKLDN GGYYITTRAQFETLQQLVQHYSERA AGLCCRLVVPCHKGMPRLTDLSVKT KDVWEIPRESLQLIKRLGNG QFGEVWMGTWNGNTKVAIKTLKPGT MSPESFLEEAQIMKKLKHDKLVQLY AVVSEEPIYIVTEYMNKGSL LDFLKDGEGRALKLPNLVDMAAQVA AGMAYIERMNYIHRDLRSANILVGN GLICKIADFGLARLIEDNEY TARQGAKFPIKWTAPEAALYGRFTI KSDVWSFGILLTELVTKGRVPYPGM NNREVLEQVERGYRMPCPQD
CPISLHELMIHCWKKDPEERPTFEY LQSFLEDYFTATEPQYQPGENLTRT RPLEQKLISEEDLAANDILDYKDDD DKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining.
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade.
Concentration : >50 ug/mL as determined by microplate BCA method.
Preparation : Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Families : Druggable Genome, Protein Kinase
Protein Pathways : Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis
Gene Name : FYN FYN proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
Official Symbol : FYN
Synonyms : SLK; SYN; p59-FYN
Gene ID : 2534
mRNA Refseq : NM_002037.5
Protein Refseq : NP_002028.1
MIM : 137025
UniProt ID : P06241

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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