Active Recombinant Full Length Human FYN Protein, C-Flag-tagged

• Cat.No.: FYN-822HFL
• Product Overview: Recombinant Full Length Human FYN Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: FYN activity verified in a biochemical assay: FYN (FYN oncogene related to SRC, FGR, YES) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FYN is a non-receptor tyrosine kinase and is a member of the Src kinase family consisting of Src, Fyn, Yes, Lck, Lyn, Hck, Fgr and Blk. Varying concentrations of FYN were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “&DeltaR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
• Molecular Mass: 60.6 kDa
• AA Sequence: MGCVQCKDKEATKLTEERDGSLNQS SGYRYGTDPTPQHYPSFGVTSIPNY NNFHAAGGQGLTVFGGVNSS SHTGTLRTRGGTGVTLFVALYDYEA RTEDDLSFHKGEKFQILNSSEGDWW EARSLTTGETGYIPSNYVAP VDSIQAEEWYFGKLGRKDAERQLLS FGNPRGTFLIRESETTKGAYSLSIR DWDDMKGDHVKHYKIRKLDN GGYYITTRAQFETLQQLVQHYSERA AGLCCRLVVPCHKGMPRLTDLSVKT KDVWEIPRESLQLIKRLGNG QFGEVWMGTWNGNTKVAIKTLKPGT MSPESFLEEAQIMKKLKHDKLVQLY AVVSEEPIYIVTEYMNKGSL LDFLKDGEGRALKLPNLVDMAAQVA AGMAYIERMNYIHRDLRSANILVGN GLICKIADFGLARLIEDNEY TARQGAKFPIKWTAPEAALYGRFTI KSDVWSFGILLTELVTKGRVPYPGM NNREVLEQVERGYRMPCPQD
  CPISLHELMIHCWKKDPEERPTFEY LQSFLEDYFTATEPQYQPGENLTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adherens junction, Axon guidance, Fc epsilon RI signaling pathway, Focal adhesion, Natural killer cell mediated cytotoxicity, Pathogenic Escherichia coli infection, Prion diseases, T cell receptor signaling pathway, Viral myocarditis

Gene Information

• Gene Name: FYN FYN proto-oncogene, Src family tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: FYN
• Synonyms: SLK; SYN; p59-FYN
• Gene ID: 2534
• mRNA Refseq: NM_002037.5
• Protein Refseq: NP_002028.1
• MIM: 137025
• UniProt ID: P06241

Reviews

07/27/2020

the manufacturer fosters scientific collaboration and knowledge sharing among researchers.

02/27/2020

They can offer insights into interpreting results and assist in identifying trends or patterns that may be relevant to the specific research question.

12/23/2019

This guarantees a continuous and uninterrupted availability of the protein for researchers, eliminating concerns about potential shortages or delays.

Q&As

How does FYN protein affect synaptic plasticity?

FYN protein is involved in regulating synaptic plasticity, which plays a crucial role in learning and memory.

Is FYN protein involved in cancer biology?

Yes, FYN protein is also associated with cancer, particularly in regulating cell proliferation and survival.

Can targeting FYN protein have applications in autoimmune diseases?

Targeting FYN protein is being studied for its potential applications in the treatment of autoimmune diseases to modulate immune responses.

Are there any targeted therapies that inhibit FYN protein for cancer treatment?

Some targeted therapies aimed at inhibiting FYN protein have been explored for specific types of cancer.

Can FYN protein levels be measured in clinical settings?

Yes, FYN protein levels can be measured in clinical settings through various laboratory techniques.