Active Recombinant Full Length Human MAP3K20 Protein, GST-tagged

• Cat.No.: MAP3K20-6867HF
• Product Overview: Full-length human ZAK [1-455(end) amino acids of accession number NP_598407.1] was expressed as N- terminal GST-fusion protein (79 kDa) using baculovirus expression system. GST-ZAK was purified by using glutathione sepharose chromatography and gel filtration chromatography.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: GST
• Protein Length: 1-455
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein/peptide, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin-coated plate. Phosphorylation was detected by anti- phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG).
• Purity: 63%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: mitogen-activated protein kinase kinase kinase 20
• Official Symbol: MAP3K20
• Synonyms: MAP3K20;AZK;CNM6;MLK7;MLT;MLTK;MLTKalpha;MLTKbeta;MRK;SFMMP;ZAK;mlklak;pk;ZAK1;homolog;leucine;zipper;and;sterile-alpha;motif;kinase;(Dictyostelium);mixed;lineage;kinase;7
• Protein Refseq: NP_598407.1
• UniProt ID: Q9NYL2