| Description : |
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
91 kDa |
| AA Sequence : |
MSSLGASFVQIKFDDLQFFENCGGG SFGSVYRAKWISQDKEVAVKKLLKI EKEAEILSVLSHRNIIQFYGVILEP PNYGIVTEYASLGSLYDYINSNRSE EMDMDHIMTWATDVAKGMHYLHMEA PVKVIHRDLKSRNVVIAADGVLKIC DFGASRFHNHTTHMSLVGTFPWMAP EVIQSLPVSETCDTYSYGVVLWEML TREVPFKGLEGLQVAWLVVEKNERL TIPSSCPRSFAELLHQCWEADAKKR PSFKQIISILESMSNDTSLPDKCNS FLHNKAEWRCEIEATLERLKKLERD LSFKEQELKERERRLKMWEQKLTEQ SNTPLLPSFEIGAWTEDDVYCWVQQ LVRKGDSSAEMSVYASLFKENNITG KRLLLLEEEDLKDMGIVSKGHIIHF KSAIEKLTHDYINLFHFPPLIKDSG GEPEENEEKIVNLELVFGFHLKPGT GPQDCKWKMYMEMDGDEIAITYIKD VTFNTNLPDAEILKMTKPPFVMEKW IVGIAKSQTVECTVTYESDVRTPKS TKHVHSIQWSRTKPQDEVKAVQLAI QTLFTNSDGNPGSRSDSSADCQWLD TLRMRQIASNTSLQRSQSNPILGSP FFSHFDGQDSYAAAVRRPQVPIKYQ QITPVNQSRSSSPTQYGLTKNFSSL HLNSRDSGFSSGNTDTSSERGRYSD RSRNKYGRGSISLNSSPRGRYSGKS QHSTPSRGRYPGKFYRVSQSALNPH QSPDFKRSPRDLHQPNTIPGMPLHP ETDSRASEEDSKVSEGGWTKVEYRK KPHRPSPAKTNKERARGDHRGWRNF TRTRPLEQKLISEEDLAANDILDYK DDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
