| Description : |
The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Bio-activity : |
PDGFRB activity verified in a biochemical assay: PDGFRB (platelet-derived growth factor receptor, beta polypeptide) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. PDGFRB is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. Varying concentrations of PDGFRB were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors. |
| Molecular Mass : |
123.8 kDa |
| AA Sequence : |
MRLPGAMPALALKGELLLLSLLLLL EPQISQGLVVTPPGPELVLNVSSTF VLTCSGSAPVVWERMSQEPP QEMAKAQDGTFSSVLTLTNLTGLDT GEYFCTHNDSRGLETDERKRLYIFV PDPTVGFLPNDAEELFIFLT EITEITIPCRVTDPQLVVTLHEKKG DVALPVPYDHQRGFFGIFEDRSYIC KTTIGDREVDSDAYYVYRLQ VSSINVSVNAVQTVVRQGENITLMC IVIGNEVVNFEWTYPRKESGRLVEP VTDFLLDMPYHIRSILHIPS AELEDSGTYTCNVTESVNDHQDEKA INITVVESGYVRLLGEVGTLQFAEL HRSRTLQVVFEAYPPPTVLW FKDNRTLGDSSAGEIALSTRNVSET RYVSELTLVRVKVAEAGHYTMRAFH EDAEVQLSFQLQINVPVRVL ELSESHPDSGEQTVRCRGRGMPQPN IIWSACRDLKRCPRELPPTLLGNSS EEESQLETNVTYWEEEQEFE VVSTLRLQHVDRPLSVRCTLRNAVG QDTQEVIVVPHSLPFKVVVISAILA LVVLTIISLIILIMLWQKKP RYEIRWKVIESVSSDGHEYIYVDPM QLPYDSTWELPRDQLVLGRTLGSGA FGQVVEATAHGLSHSQATMK VAVKMLKSTARSSEKQALMSELKIM SHLGPHLNVVNLLGACTKGGPIYII TEYCRYGDLVDYLHRNKHTF LQHHSDKRRPPSAELYSNALPVGLP LPSHVSLTGESDGGYMDMSKDESVD YVPMLDMKGDVKYADIESSN YMAPYDNYVPSAPERTCRATLINES PVLSYMDLVGFSYQVANGMEFLASK NCVHRDLAARNVLICEGKLV KICDFGLARDIMRDSNYISKGSTFL PLKWMAPESIFNSLYTTLSDVWSFG ILLWEIFTLGGTPYPELPMN EQFYNAIKRGYRMAQPAHASDEIYE IMQKCWEEKFEIRPPFSQLVLLLER LLGEGYKKKYQQVDEEFLRS DHPAILRSQARLPGFHGLRSPLDTS SVLYTAVQPNEGDNDYIIPLPDPKP EVADEGPLEGSPSLASSTLN
EVNTSSTISCDSPLEPQDEPEPEPQ LELQVEPEPELEQLPDSGCPAPRAE AEDSFLTRTRPLEQKLISEEDLAAN DILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane |
| Protein Pathways : |
Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
