Active Recombinant Full Length Human PICALM Protein, C-Flag-tagged

• Cat.No.: PICALM-606HFL
• Product Overview: Recombinant Full Length Human PICALM Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(10;11)(p13;q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: In vitro binding assay
• Molecular Mass: 70.6 kDa
• AA Sequence: MSGQSLTDRITAAQHSVTGSAVSKT VCKATTHEIMGPKKKHLDYLIQCTN EMNVNIPQLADSLFERTTNS SWVVVFKSLITTHHLMVYGNERFIQ YLASRNTLFNLSNFLDKSGLQGYDM STFIRRYSRYLNEKAVSYRQ VAFDFTKVKRGADGVMRTMNTEKLL KTVPIIQNQMDALLDFNVNSNELTN GVINAAFMLLFKDAIRLFAA YNEGIINLLEKYFDMKKNQCKEGLD IYKKFLTRMTRISEFLKVAEQVGID RGDIPDLSQAPSSLLDALEQ HLASLEGKKIKDSTAASRATTLSNA VSSLASTGLSLTKVDEREKQAALEE EQARLKALKEQRLKELAKKP HTSLTTAASPVSTSAGGIMTAPAID IFSTPSSSNSTSKLPNDLLDLQQPT FHPSVHPMSTASQVASTWGD PFSATVDAVDDAIPSLNPFLTKSSG DVHLSISSDVSTFTTRTPTHEMFVG FTPSPVAQPHPSAGLNVDFE SVFGNKSTNVIVDSGGFDELGGLLK PTVASQNQNLPVAKLPPSKLVSDDL DSSLANLVGNLGIGNGTTKN DVNWSQPGEKKLTGGSNWQPKVAPT TAWNAATMAPPVMAYPATTPTGMIG YGIPPQMGSVPVMTQPTLIY
  SQPVMRPPNPFGPVSGAQIQFMTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: PICALM phosphatidylinositol binding clathrin assembly protein [ Homo sapiens (human) ]
• Official Symbol: PICALM
• Synonyms: LAP; CALM; CLTH
• Gene ID: 8301
• mRNA Refseq: NM_007166.4
• Protein Refseq: NP_009097.2
• MIM: 603025
• UniProt ID: Q13492