Active Recombinant Full Length Human POU5F1 Protein, C-Flag-tagged

• Cat.No.: POU5F1-327HFL
• Product Overview: Recombinant Full Length Human POU5F1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: EMSA assay
• Molecular Mass: 38.4 kDa
• AA Sequence: MAGHLASDFAFSPPPGGGGDGPGGP EPGWVDPRTWLSFQGPPGGPGIGPG VGPGSEVWGIPPCPPPYEFC GGMAYCGPQVGVGLVPQGGLETSQP EGEAGVGVESNSDGASPEPCTVTPG AVKLEKEKLEQNPEESQDIK ALQKELEQFAKLLKQKRITLGYTQA DVGLTLGVLFGKVFSQTTICRFEAL QLSFKNMCKLRPLLQKWVEE ADNNENLQEICKAETLVQARKRKRT SIENRVRGNLENLFLQCPKPTLQQI SHIAQQLGLEKDVVRVWFCN RRQKGKRSSSDYAQREDFEAAGSPF SGGPVSFPLAPGPHFGTPGYGSPHF TALYSSVPFPEGEAFPPVSV
  TTLGSPMHSNTRTRPLEQKLISEED LAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Adult stem cells, Cancer stem cells, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Transcription Factors

Gene Information

• Gene Name: POU5F1 POU class 5 homeobox 1 [ Homo sapiens (human) ]
• Official Symbol: POU5F1
• Synonyms: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; Oct3/4
• Gene ID: 5460
• mRNA Refseq: NM_002701.6
• Protein Refseq: NP_002692.2
• MIM: 164177
• UniProt ID: Q01860