Active Recombinant Full Length Human TP53 Protein, C-Flag-tagged

• Cat.No.: TP53-06HFL
• Product Overview: Recombinant Full Length Human TP53 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Bio-activity: TP53 Activity Verified in a DNA-binding Assay: TP53 (TP304649) activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the purified TP53 protein and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm.
  EMSA reaction
  Pull-down assay
• Molecular Mass: 43.5 kDa
• AA Sequence: MEEPQSDPSVEPPLSQETFSDLWKL LPENNVLSPLPSQAMDDLMLSPDDI EQWFTEDPGPDEAPRMPEA APPVAPAPAAPTPAAPAPAPSWPLS SSVPSQKTYQGSYGFRLGFLHSGTA KSVTCTYSPALNKMFCQLA KTCPVQLWVDSTPPPGTRVRAMAIY KQSQHMTEVVRRCPHHERCSDSDGL APPQHLIRVEGNLRVEYLD DRNTFRHSVVVPYEPPEVGSDCTTI HYNYMCNSSCMGGMNRRPILTIITL EDSSGNLLGRNSFEVRVCA CPGRDRRTEEENLRKKGEPHHELPP GSTKRALPNNTSSSPQPKKKPLDGE YFTLQIRGRERFEMFRELN
  EALELKDAQAGKEPGGSRAHSSHLK SKKGQSTSRHKKLMFKTEGPDSDTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway

Gene Information

• Gene Name: TP53 tumor protein p53 [ Homo sapiens (human) ]
• Official Symbol: TP53
• Synonyms: BCC7; BMFS5; LFS1; P53; TRP53
• Gene ID: 7157
• mRNA Refseq: NM_000546.6
• Protein Refseq: NP_000537.3
• MIM: 191170
• UniProt ID: P04637