Active Recombinant Human Aminoacyl TRNA Synthetase Complex-Interacting Multifunctional Protein 1

• Cat.No.: AIMP1-338H
• Product Overview: Recombinant human AIMP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Description: EMAP-II is a tumor derived cytokine that exerts a wide range of activities on endothelial cells, monocytes and neutrophils. EMAP-II inhibits endothelial cell proliferation, vasculogenesis, neovessel formation, and can induce apoptosis. It is also chemotactic towards neutrophils and monocytes and induces myeloperoxidase activity from neutrophils. Of clinical importance, EMAP-II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues. Mature EMAP-II is an 18.3 kDa protein, which is synthesized as the C-terminal portion of a biologically inactive precursor protein containing a propeptide of 146 amino acid residues.
• Form: Supplied as a lyophilized powder.
• Purity: >98% pure by SDS PAGE and HPLC
• Sequence: Region of EMAP II protein corresponding to amino acids SKPIDVSRLD LRIGCIITAR KHPDADSLYV EEVDVGEIAP RTVVSGLVNH VPLEQMQNRM VILLCNLKPA KMRGVLSQAM VMCASSPEKI EILAPPNGSV PGDRITFDAF PGEPDKELNP KKKIWEQIQP DLHTNDECVA TYKGVPFEVK GKGVCRAQTM SNSGIK.
• Molecular Mass: 18.3 kDa
• Applications: Numerous applications are possible with this product and should be tested by the end user under their own laboratory conditions
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Bioactivity: Determined by the apoptotic effect on MCF-7 cells using a concentration of 20-40 ng/ml.
• Storage: Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Gene Information

• Gene Name: AIMP1 aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 [ Homo sapiens ]
• Official Symbol: AIMP1
• Synonyms: AIMP1; SCYE1; EMAP II; EMAP-2; EMAP-II; EMAP2; EMAPII; p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); small inducible cytokine subfamily E, member 1; OTTHUMP00000161878; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; endothelial-monocyte activating polypeptide II; multisynthetase complex auxiliary component p43
• Gene ID: 9255
• mRNA Refseq: NM_001142415
• Protein Refseq: NP_001135887
• MIM: 603605
• UniProt ID: Q12904
• Chromosome Location: 4q2
• Pathway: Cytosolic tRNA aminoacylation; Gene Expression; tRNA Aminoacylation
• Function: cell surface binding; cytokine activity; protein binding; protein homodimerization activity; tRNA binding.