Active Recombinant Human ANPEP protein(603 Met/Ile), His tagged

• Cat.No.: ANPEP-249H
• Product Overview: Active Recombinant Human ANPEP protein(Lys 69-Lys 967，603 Met/Ile)(NP_001141.2) was expressed in Human Cells with N-terminal signal peptide and C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 69-967 aa
• Form: Lyophilized from sterile PBS, pH 7.3.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Ala-7-amido-4-methylcoumarin (Ala-AMC). The specific activity is >2,500 pmoles/min/μg.
• Molecular Mass: The recombinant human ANPEP protein consists of 910 amino acids and has a predicted molecular mass of 104.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhANPEP is approximately 135-140 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ANPEP alanyl (membrane) aminopeptidase [ Homo sapiens ]
• Official Symbol: ANPEP
• Synonyms: ANPEP; alanyl (membrane) aminopeptidase; CD13, PEPN; aminopeptidase N; aminopeptidase M; gp150; LAP1; microsomal aminopeptidase; p150; AP-M; AP-N; hAPN; alanyl aminopeptidase; myeloid plasma membrane glycoprotein CD13; APN; CD13; P150; PEPN; GP150
• Gene ID: 290
• mRNA Refseq: NM_001150
• Protein Refseq: NP_001141
• MIM: 151530
• UniProt ID: P15144