Active Recombinant Human ASGR1 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ASGR1-251H
• Product Overview: Biotinylated Recombinant Human ASGR1 protein(Gln 62 - Leu 291 )(P07306-1), fused with His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Biotinylated Human ASGR1 / ASGPR1, His,Avitag on SA Biosensor, can bind SARS-CoV-2 , S1 protein, His tag with an affinity constant of 79.2 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: The protein has a calculated MW of 33.4 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Gln 62 - Leu 291
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended to reconstitute the lyophilized powder in sterile water to 100-200 ug/ml, and further dilute with other solvents. The dissolved protein can be stored at 4°C for 2-7 days and should be used up as soon as possible. If not in use for a short time, please store at -20°C.

Gene Information

• Gene Name: ASGR1 asialoglycoprotein receptor 1 [ Homo sapiens ]
• Official Symbol: ASGR1
• Synonyms: ASGR1; asialoglycoprotein receptor 1; CLEC4H1; hepatic lectin H1; C-type lectin domain family 4 member H1; HL-1; ASGPR; ASGPR1
• Gene ID: 432
• mRNA Refseq: NM_001197216
• Protein Refseq: NP_001184145
• MIM: 108360
• UniProt ID: P07306