Active Recombinant Human ATXN3L, His-tagged

• Cat.No.: ATXN3L-174H
• Product Overview: Recombinant human ATXN3L (amino acid residues 1-355), fused with N-terminal His, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-355 a.a.
• Description: There are two main classes of DUB; cysteine proteases and metalloproteases. Ataxin-3L is a cysteine protease and a member of the Machado-Joseph Domain (MJD) enzyme family. Cloning of the human gene was first described by Gerhard et al. (2004). Machado–Joseph disease (MJD), the most common form of spinocerebellar ataxia worldwide, is a progressive and ultimately fatal neurodegenerative disorder caused by polyQ expansion in ataxin-3, a conserved and ubiquitous protein known to bind polyubiquitin chains and to function as a deubiquitylating enzyme.
• Form: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
• Bio-activity: Deubiquitylase Enzyme Assay: The activity of His-Ataxin-3L was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of His-Ataxin-3L was compared confirming the deubiquitylating activity of His-Ataxin-3L.
• Molecular Mass: ~43 kDa
• Purity: >92% by SDS-PAGE
• Storage: 12 months at -70°C. Avoid multiple freeze/thaw cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: ATXN3L ataxin 3-like [ Homo sapiens ]
• Official Symbol: ATXN3L
• Synonyms: ataxin 3-like; ATX3L; MJDL; EC 3.4.19.12; Machado-Joseph disease protein 1-like; EC 3.4.22; putative ataxin-3-like protein
• Gene ID: 92552
• mRNA Refseq: NM_001135995.1
• Protein Refseq: NP_001129467.1
• UniProt ID: B4DYC7
• Chromosome Location: Xp22.2
• Pathway: Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem
• Function: omega peptidase activity; ubiquitin-specific protease activity