Active Recombinant Human BACE2, His-tagged

• Cat.No.: BACE2-579TH
• Product Overview: Recombinant soluble BACE2, cloned from human cDNA, secreted as zymogen from insect cells, purified using a C-terminal His-tag, and cleaved to the mature active form.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: This gene encodes an integral membrane glycoprotein that functions as an aspartic protease. The encoded protein cleaves amyloid precursor protein into amyloid beta peptide, which is a critical step in the etiology of Alzheimer''s disease and Down syndrome. The protein precursor is further processed into an active mature peptide. Alternative splicing results in multiple transcript variants.
• Form: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 20% glycerol.
• Bio-activity: >30 U/μg enzyme.
• Molecular Mass: ~45kDa (calculated); ~48kDa (doublet by SDS-PAGE)
• AA Sequence: aa Ala21-Pro466.
• Purity: ≥80% (SDS-PAGE)
• Applications: Study enzyme kinetics, cleave target substrates, and screen for BACE2 inhibitors or BACE1 inhibitor selectivity.
• Stability: BACE2 is stable after 5 freeze-thaws at 0.3-0.4 µg/µl; freeze-thaw stability of more dilute preparations has not been tested and could lead to loss of activity.
• Storage: -80°C

Gene Information

• Gene Name: BACE2 beta-site APP-cleaving enzyme 2 [ Homo sapiens (human) ]
• Official Symbol: BACE2
• Synonyms: BACE2; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; beta-site APP-cleaving enzyme 2; beta-secretase 2; memapsin-1; theta-secretase; aspartyl protease 1; 56 kDa aspartic-like protease; Down syndrome region aspartic protease; transmembrane aspartic proteinase Asp1; membrane-associated aspartic protease 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme 2; EC 3.4.23.45
• Gene ID: 25825
• mRNA Refseq: NM_012105
• Protein Refseq: NP_036237
• MIM: 605668
• UniProt ID: Q9Y5Z0
• Chromosome Location: 21q22.3
• Pathway: Alzheimer''s disease
• Function: aspartic-type endopeptidase activity