Active Recombinant Human BID protein

• Cat.No.: BID-3838H
• Product Overview: Active Recombinant Human BID protein(P55957-1) (Met 1-Asp 195) was expressed and purified in E. coli, with additional two amino acids (Gly & Pro) at the N-terminus.

Specification

• Species: Human
• Source: E. coli
• Tag: Non
• Protein Length: 1-195 aa
• Form: Lyophilized from sterile 40mM Tris, 150mM NaCl, pH 8.0.
• Bio-activity: Measured by its binding ability in a functional ELISA. 1. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL. 2. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.
• Molecular Mass: The recombinant human BID consists of 197 amino acids and migrates as an approximately 22 kDa band in SDS-PAGE under reducing conditions as predicted.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BID BH3 interacting domain death agonist [ Homo sapiens ]
• Official Symbol: BID
• Synonyms: BID; BH3 interacting domain death agonist; BH3-interacting domain death agonist; p22 BID; BID isoform Si6; BID isoform L(2); BID isoform ES(1b); desmocollin type 4; apoptic death agonist; Human BID coding sequence; FP497; MGC15319; MGC42355
• Gene ID: 637
• mRNA Refseq: NM_001196
• Protein Refseq: NP_001187
• MIM: 601997
• UniProt ID: P55957